酶水解法合成多肽鐵螯合物（Polypeptide iron chelate）

酶水解法利用單一酶或者復(fù)合酶在最適條件下對(duì)大豆蛋白、蝦蛋白、鰱魚蛋白等食物蛋白質(zhì)進(jìn)行水解，最終制得多肽。相較于單酶水解法，復(fù)合酶水解法在制備多肽時(shí)既可以提高水解度，又能在短時(shí)間內(nèi)降低酶解產(chǎn)物的分子量，因此當(dāng)前多用復(fù)合酶水解的方法制備多肽，常用于制備亞鐵螯合肽的酶有中性蛋白酶、胰蛋白酶、堿性蛋白酶等。然而，生物酶價(jià)格昂貴，為達(dá)到最佳水解結(jié)果在工藝上要嚴(yán)格控制反應(yīng)條件，同時(shí)避免與具有復(fù)雜基質(zhì)食物蛋白質(zhì)的相互作用。目前關(guān)于這方面的相關(guān)研究較少，還有待進(jìn)一步探索。微生物發(fā)酵法是利用微生物菌種代謝產(chǎn)生的蛋白酶水解底物蛋白，繼而從發(fā)酵液中制備多肽產(chǎn)物的方法。雖然發(fā)酵法生產(chǎn)金屬離子螯合肽仍處于起步階段，但因其安全、經(jīng)濟(jì)、環(huán)保的優(yōu)勢(shì)受到越來越多的關(guān)注。發(fā)酵菌株具有安全性高、產(chǎn)酶豐富、生長(zhǎng)迅速的優(yōu)點(diǎn)，與酶水解法相比，微生物發(fā)酵生產(chǎn)多肽的成本相對(duì)較低。然而，微生物發(fā)酵法應(yīng)用范圍較窄，使生產(chǎn)多肽的發(fā)酵工業(yè)受到阻礙。因此，在工業(yè)生產(chǎn)中常將微生物發(fā)酵法與酶水解法相結(jié)合以提高生產(chǎn)效率，從而達(dá)到高效制備金屬離子螯合肽的目的。

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抗壞血酸亞鐵Ferrous Ascorbate、賴氨酸甘氨酸鎂Magnesium Lysinate Glycinate、甘氨酸谷氨酰胺鎂Magnesium Glycinate Glutamine、檸檬酸蘋果酸鎂Magnesium Citrate Malate、檸檬酸鍶Strontium Citrate、檸檬酸錳Manganese Citrate、檸檬酸銅Copper Citrate、天門冬氨酸鋰Lithium Aspartate、抗壞血酸錳Manganese Ascorbate、?；撬嵛?/span>Selenium Taurate。

Synthesis of polypeptide iron chelate by enzymatic hydrolysis

Enzymatic hydrolysis method uses single enzyme or complex enzyme to hydrolyze soybean protein, shrimp protein, silver carp protein and other food proteins under optimal conditions, and finally produces polypeptides. Compared with the single enzymatic hydrolysis method, the composite enzymatic hydrolysis method can not only improve the degree of hydrolysis, but also reduce the molecular weight of the enzymatic hydrolysis products in a short time. Therefore, the composite enzymatic hydrolysis method is commonly used to prepare peptides. The enzymes commonly used to prepare ferrous chelate peptides include neutral protease, trypsin, alkaline protease and so on. However, biological enzymes are expensive, and in order to achieve the best hydrolysis results, the reaction conditions must be strictly controlled in the process, while avoiding interaction with food proteins with complex substrates. At present, there are few relevant studies on this aspect, and it needs to be further explored. Microbial fermentation is a method of hydrolyzing substrate protein with protease produced by microbial strain metabolism, and then preparing polypeptide products from fermentation solution. Although the production of metal ion chelate peptides by fermentation is still in its infancy, it has attracted more and more attention because of its advantages of safety, economy and environmental protection. The fermentation strain has the advantages of high safety, abundant enzyme production and rapid growth. Compared with enzymatic hydrolysis method, the cost of microbial fermentation to produce polypeptides is relatively low. However, the application of microbial fermentation method is narrow, which hinders the fermentation industry for the production of peptides. Therefore, in industrial production, microbial fermentation and enzymatic hydrolysis are often combined to improve production efficiency, so as to achieve the purpose of efficient preparation of metal ion chelating peptides.Company's official website: www.wilincare.net welcome to buy!

Ferrous Ascorbate, Magnesium Lysinate Glycinate, Magnesium lysinate Glycinate Glutamine, Magnesium citrate malate Citrate Malate, Strontium Citrate, Manganese Citrate, Copper Citrate, Lithium Aspartate, Manganese ascorbate Ascorbate, Selenium Taurate.